[Histonet] MMA sections coming off!

Joanne Mauger mauger <@t> email.chop.edu
Tue Dec 20 08:41:50 CST 2005


I should also say we are doing regular paraffin blocks for routine
histology and Immuno.
Jo

>>> "Patricia F Lott" <plott <@t> uab.edu> 12/19/05 3:21 PM >>>
Help!  We are losing our thin plastic sections!  We have cut PMMA
plastic sections at 5 microns and put them on "+" slides, and the
sections fall off either during de-plasticizing or during staining! 
We
have tried:  Haupt's adhesive, longer drying times, "+" slides, plus
slides and Haupt's together, etc....no luck!  Any suggestions would be
welcome!
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