[Histonet] Re:Sections coming off!

Joanne Mauger mauger <@t> email.chop.edu
Tue Dec 20 08:39:43 CST 2005


Hi Patricia,

Are you using slides from Erie? We buy ours through Fisher, and have
been having trouble keeping sections on + slides as well. It is as if
they have no charge at all. We think the lot may be bad. The company
claims to have no complaints.

Anyone else having similar problem?
Jo Mauger 

>>> "Patricia F Lott" <plott <@t> uab.edu> 12/19/05 3:21 PM >>>
Help!  We are losing our thin plastic sections!  We have cut PMMA
plastic sections at 5 microns and put them on "+" slides, and the
sections fall off either during de-plasticizing or during staining! 
We
have tried:  Haupt's adhesive, longer drying times, "+" slides, plus
slides and Haupt's together, etc....no luck!  Any suggestions would be
welcome!
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