[Histonet] Dako streptavidin / FITC for immuno's

[PALMER Jason (SVHM)]

Dear all,

Just wondering if anybody has used Dako streptavidin / FITC (F0422) in an immunohisto or immunocytochemistry run, and roughly what dilution you found to be best? We have very limited material to work with and so can't really do a titration to find the best dilution for ourselves (which is what we would normally do). It is to be used with a Stro-1 primary and biotinylated goat anti mouse secondary on cultured cells. The spec sheet has a suggested dilution range for flow cytometry only. Much obliged if anyone can help.

Cheers!

Jason

Jason Palmer
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: palmerj <@t> svhm.org.au




Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies.