[Histonet] anti-human VEGF antibody

[pruegg <@t> ihctech.net]

I am using mouse anti-vegf from Santa Cruz with good results on human and
baboon ffpe tissues, it is not easy, but I do HIER in a water bath in high
ph buffer at 85dc for 3 hrs., followed by overnight incubation of the
primary 1:200, detected with mouse labeled polymer (I use Envision from
DAKO).
Patsy

> Andrea,
> I cannot say this is the best anti-VEGF antibody, but I am doing a
> research
> project on FFPE human brain tissue and using placenta as a control with
> following antibody:
> monoclonal mouse anti human VEGF, clone VG1 from ID Labs (www.idlabs.com),
> dilution 1:100, HIER in citrate buffer pH 6.0. Detection system is from
> Zymed, Histostain Plus Kit..
> Glioblastoma stains strongly with this antibody. I have not used this with
> frozen sections.
>
> Katri
>
> Katri Tuomala
> Hamilton, Ontario, Canada
> ----- Original Message -----
> From: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Monday, December 19, 2005 8:37 PM
> Subject: [Histonet] anti-human VEGF antibody
>
>
>> What's the current consensus on the best antibody to use for anti-human
>> VEGF immunostaining on human FFPE sections (frozens as well if
>> possible).
>>
>> Thanks so much!!
>> Andrea
>> --
>>
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