[Histonet] Tissue Proccessing HELP!!!!!!!!!!!!!!

[Katri Tuomala]

Jesus,
If the biopsy is unreadable, there is nothing to lose to
follow Carole's suggestion. Obviously you'll have to melt the paraffin block 
and the specimen first, remove the wax with xylene and bring it through to a 
low cocentration alcohol (50%) before putting it in the sodium carbonate 
solution.

Good luck!

Katri

Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Carole Fields" <cgfields <@t> lexhealth.org>
To: "'Jesus Ellin'" <JEllin <@t> yumaregional.org>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, December 14, 2005 8:20 AM
Subject: RE: [Histonet] Tissue Proccessing HELP!!!!!!!!!!!!!!


> We had a problem with a biopsy being left in the cleaning cycle of the
> processor and found the next day.  It looked like a rock.  We put it in
> Ruffer's Solution.
>
> 5% Sodium Carbonate Sol.
>
> 0.6 gm Sodium Carbonate to 42 ml. H2O
> 18 ml. Absolute Ethanol
>
> Mix Sod. Carb. Solution and add 18 ml Alcohol mixture
>
> Place tissue in solution for 8 hours or longer depending on the size of 
> the
> tissue.  Then proceed to processing tissue through alcohols.  The alcohols
> will refix the tissue.  Finish processing and embedding in paraffin.
>
> We left the tissue 8-12 hours washed it and reprocessed it on the VIP.  It
> was not totally perfect but readable.  I found this procedure on
> histonet <@t> pathology.swmed.edu.  Gayle Callis posted this and she was at
> Montana State University, Bozeman, MT, 404-994-4705.
>
> Good luck,
> Carole Fields, HT,ASCP
> Pathology Supervisor
> Lexington Medical Center
> 2720 Sunset Blvd.
> W. Columbia, SC 29169
>
>
>
>
>
> -----Original Message-----
> From: Jesus Ellin [mailto:JEllin <@t> yumaregional.org]
> Sent: Tuesday, December 13, 2005 8:29 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Tissue Proccessing HELP!!!!!!!!!!!!!!
>
>
> Hello everyone I have a real problem here, We have just accquired a tissue
> proccesor (VIP 5) and found out the hard way like everything in Histology,
> there is a problem with the proccessing.  Our pathologist are claiming 
> that
> the small biopsies are coming out cooked,  after reviewing the slide 
> myself
> with the pathologist it is not a cooked looked but rather a glassy look 
> and
> bubbly.  Later found out that the latch of the tissue proccessor is not
> tight enough and is was allowing moisture in and there was a lot of
> condensation.  I figured out what was wrong but here is the kicker.
>
> My Pathologist is wanting to take one of the small biopsies that was
> proccessed and regress the tissue and then reproccess the small biopsy by
> hand instead of throwing it back on the tissue proccessor.  The biopsy is
> about .1 mm very tiny.  I need all the help that I can get!!!!!!  So 
> please
> fellow histo-netters share the knowledge with me.
>
>
> Jesus Ellin
> Yuma Regional Medical Center
>
>
>
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