[Histonet] re: rat spinal cord longitudinal sections

[Tracey Couse]

Carolyn,

I agree with you.  I would section longitudinally so as to limit the 
section number and time analyzing, especially if this is preliminary 
work and you are simply screening.  I work with graduates students who 
currently perform similar studies in the sense that they are looking for 
a fluorescent signal in rat/mouse spinal cord and many of them section 
longitudinally.

Good luck!
Tracey

-- 
Tracey Couse
Laboratory Coordinator
Georgia Tech/Emory Center for the
      Engineering of Living Tissues
Georgia Institute of Technology
Office: 404.385.2611
Lab: 404-385-6735
Fax: 404.894.2291


From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
 > Caroline Bass
 > Sent: 	Monday, December 12, 2005 2:02 PM
 > To: 	Histonet (E-mail)
 > Subject: 	[Histonet] rat spinal cord longitudinal sections
 >
 > Hi Guys,
 >
 > I have a rat that was injected with a virus that produces a
 > fluorescent protein.  I would like to scan the entire spinal cord for
 > the signal and thought the best way to do this was to section the
 > spinal cord along the long axis.  I don't know if I have the
 > terminology right on this.  I think this should be longitudinal
 > sections.  The histologists who is to section the tissue says that
 > this is impractical.  I know that cross sections (coronal?) are more
 > common, but which method is better when we are simply screening for a
 > visible signal?  I am worried that I will have hundreds of cross
 > sections to examine.
 >
 > If there is a published example of this anywhere, even a picture on a
 > website I would love to see it.  I have not been able to find
 > anything that is helpful in deciding which route to go.
 >
 > Thanks for you help,
 >
 > Caroline Bass