[Histonet] Tissue Proccessing HELP!!!!!!!!!!!!!!

[Carole Fields]

We had a problem with a biopsy being left in the cleaning cycle of the
processor and found the next day.  It looked like a rock.  We put it in
Ruffer's Solution.

5% Sodium Carbonate Sol.

0.6 gm Sodium Carbonate to 42 ml. H2O
18 ml. Absolute Ethanol

Mix Sod. Carb. Solution and add 18 ml Alcohol mixture

Place tissue in solution for 8 hours or longer depending on the size of the
tissue.  Then proceed to processing tissue through alcohols.  The alcohols
will refix the tissue.  Finish processing and embedding in paraffin.

We left the tissue 8-12 hours washed it and reprocessed it on the VIP.  It
was not totally perfect but readable.  I found this procedure on
histonet <@t> pathology.swmed.edu.  Gayle Callis posted this and she was at
Montana State University, Bozeman, MT, 404-994-4705.

Good luck,
Carole Fields, HT,ASCP
Pathology Supervisor
Lexington Medical Center
2720 Sunset Blvd.
W. Columbia, SC 29169





-----Original Message-----
From: Jesus Ellin [mailto:JEllin <@t> yumaregional.org]
Sent: Tuesday, December 13, 2005 8:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue Proccessing HELP!!!!!!!!!!!!!!


Hello everyone I have a real problem here, We have just accquired a tissue
proccesor (VIP 5) and found out the hard way like everything in Histology,
there is a problem with the proccessing.  Our pathologist are claiming that
the small biopsies are coming out cooked,  after reviewing the slide myself
with the pathologist it is not a cooked looked but rather a glassy look and
bubbly.  Later found out that the latch of the tissue proccessor is not
tight enough and is was allowing moisture in and there was a lot of
condensation.  I figured out what was wrong but here is the kicker.

My Pathologist is wanting to take one of the small biopsies that was
proccessed and regress the tissue and then reproccess the small biopsy by
hand instead of throwing it back on the tissue proccessor.  The biopsy is
about .1 mm very tiny.  I need all the help that I can get!!!!!!  So please
fellow histo-netters share the knowledge with me.


Jesus Ellin
Yuma Regional Medical Center



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