[Histonet] DAPI vs. Hoescht
cbass <@t> bidmc.harvard.edu
Wed Dec 14 14:16:23 CST 2005
I would like to stain my sections with something to highlight the
nucleus. I have heard that both DAPI and Hoescht are good. Is there
are reason to use one over the other? I am looking for something
very quick, easy and inexpensive. I know that Molecular Probes makes
an anti-fade with DAPI, but this is rather expensive and I am worried
about not being able to optimize the conditions.
I am mainly interested in hearing info on the best formulation to
use, a reliable company to purchase from, and any simple protocol you
may have on hand.
I am staining liver sections, 10 micron, NBF fixed, cryoprotected.
More information about the Histonet