[Histonet] DAPI vs. Hoescht

[Caroline Bass]

Hello,

I would like to stain my sections with something to highlight the  
nucleus.  I have heard that both DAPI and Hoescht are good.  Is there  
are reason to use one over the other?  I am looking for something  
very quick, easy and inexpensive.  I know that Molecular Probes makes  
an anti-fade with DAPI, but this is rather expensive and I am worried  
about not being able to optimize the conditions.

I am mainly interested in hearing info on the best formulation to  
use, a reliable company to purchase from, and any simple protocol you  
may have on hand.

I am staining liver sections, 10 micron, NBF fixed, cryoprotected.

Thanks,

Caroline Bass