[Histonet] non-specific staining IHC

[Mrosla, Tina M]

Hello,
Our lab has recently noticed non-specific staining on our negative antibodies (mouse and rabbit) since we changed our protocols to comply with CAP guidelines. We apply our harshest pre-treatments to our negative controls, including cell conditioner, protease, blocker, and amplification kits. We have a Ventana BenchMark and use their products. I have never experienced background staining before, so I need some advice. Is this a common problem and can it be fixed?
Thank you!

Tina Mrosla
St. Joseph's Hospital 
St. Paul, MN


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