[Histonet] RE: Paraffin sections
Andrea T. Hooper
anh2006 <@t> med.cornell.edu
Fri Dec 2 08:56:52 CST 2005
Very interesting Chris! I have found sometimes this works and
sometimes it doesn't and you get complete loss of staining -
frustrating when one was just trying to save time to begin with!! I
imagine it's antigen dependent but I have not had time to check.
In my opinion Betsy, if you can avoid it (because you are just doing
it to save time instead of setting up a workshop) then avoid it ...
it simply isn't worth the risk IMHO.
At 9:52 AM +0100 12/2/05, C.M. van der Loos wrote:
> For my NSH wet-workshops I tested the option of going up to endogenous
> PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.
> Next day we started at the protein blocking step and then the rest.
> Staining was as good as running the whole procedure in one day.
> Hope this info helps.
> Chris van der Loos, PhD
> Dept. of Pathology
> Academic Medical Center M2-230
> Meibergdreef 9
> NL-1105 AZ Amsterdam
> The Netherlands
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