[Histonet] RE: Paraffin sections
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Fri Dec 2 02:52:13 CST 2005
For my NSH wet-workshops I tested the option of going up to endogenous
PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.
Next day we started at the protein blocking step and then the rest.
Staining was as good as running the whole procedure in one day.
Hope this info helps.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Thu, 1 Dec 2005 07:46:32 -0600
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
Subject: [Histonet] Paraffin sections
To: <histonet <@t> lists.utsouthwestern.edu>
I have heard that paraffin sections that have been deparaffinized and
run down to DH20 but for some reason cannot be stained that day, can
left to air dry and then be placed back into H2O when they are ready
staining. Does anyone have any experience with this technique? I
just leave them in DH2O till I can get to them, usually the next day.
Betsy Molinari HT (ASCP)
Texas Heart Institute
6770 Bertner Ave.
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