[Histonet] RE: Paraffin sections

[C.M. van der Loos]

   Betsy,

   For my NSH wet-workshops I tested the option of going up to endogenous
   PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.
   Next  day  we  started at the protein blocking step and then the rest.
   Staining was as good as running the whole procedure in one day.

   Hope this info helps.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands



   Date: Thu, 1 Dec 2005 07:46:32 -0600
   From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
   Subject: [Histonet] Paraffin sections
   To: <histonet <@t> lists.utsouthwestern.edu>
   Hi,
    I have heard that paraffin sections that have been deparaffinized and
   run  down  to DH20 but for some reason cannot be stained that day, can
   be
   left  to  air dry and then be placed back into H2O when they are ready
   for
   staining.  Does  anyone  have  any  experience  with this technique? I
   usually
   just leave them in DH2O till I can get to them, usually the next day.
   Thanks.
   Betsy Molinari HT (ASCP)
   Texas Heart Institute
   Cardiovascular Pathology
   6770 Bertner Ave.
   Houston,TX 77030