[Histonet] coating slides for paraffin sections of CNS tissue

Favara, Cynthia (NIH/NIAID) cfavara <@t> niaid.nih.gov
Tue Apr 26 08:05:54 CDT 2005


I work with primarily rodent CNS tissue and use Superfrost/Plus I get them
from Fischer in the US Cat# 22-034-979 and very rarely have any tissue loss
generally when I neglect to properly dry the slide. Hope this is helpful.


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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-----Original Message-----
From: Julia Edgar [mailto:je22r <@t> udcf.gla.ac.uk] 
Sent: Tuesday, April 26, 2005 1:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] coating slides for paraffin sections of CNS tissue

Dear All
We've been using APES coated slides for collecting paraffin embedded CNS
tissue for immunohistochemistry (with microwave treatment) but the sections
are becoming unstuck, to some extent, during processing. I will be grateful
if you can tell me what you are coating slides with for small pieces of CNS
Thank you
Julia Edgar BSc (Hons), PhD
University of Glasgow
Tel: 0141 330 5818
e-mail: je22r <@t> udcf.gla.ac.uk

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