[Histonet] immuno-LCM

Garry Ashton GAshton <@t> PICR.man.ac.uk
Tue Apr 26 05:14:18 CDT 2005

Dear all,
I'm currently trying to re-optimize an immuno technique on frozen
sections that allows for the production of quality RNA after laser
Presently I have found that not allowing the sections to dry, storing at
-80, and using a much shortened immuno method (2 mins max in all ab
incubations), plus a shortened colour reaction, with an RNase inhibitor
added at the ab and colour steps gives me reasonable results.
Does anybody else in histoland use this technique regularly, and if so
have they found results similar to me.
Many thanks.

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