[Histonet] flourescent

[Geoff McAuliffe]

Hi Renee:

    The basic princilpes and protocols are pretty much the same for all 
immunos, if you can do cells sections should not be that much of a 
problem. Easy for me to say! Get a DAKO kit, a Vector kit, a Zymed kit 
or whatever and follow the instructions. Call the maker of the kit 
before you order to be sure you are getting the correct kit for your 
application. If possible, go to a lab that does these regularly and 
learn from someone with experience.
    Go to the DAKO USA site and download their excellent immunostaining 
manual. It will teach you everything you need and more.

Geoff

Till, Renee wrote:

>Is there a basic protocol for doing fluorescent IHC on frozen or
>paraffin sections? Is it better to use a primary antibody conjugated
>with FITC or whatever, instead of with the secondary? I never thought it
>would be such a headache figuring out fluorescence staining on sectioned
>tissues as opposed to cells. Also, do you need to get all the reagent
>separate, or can some of the detections kits be used for fluorescence?
>
>Thanks.
>
> 
>
>Renee' Till, HT
>
> 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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