[Histonet] flourescent

[Till, Renee]

Is there a basic protocol for doing fluorescent IHC on frozen or
paraffin sections? Is it better to use a primary antibody conjugated
with FITC or whatever, instead of with the secondary? I never thought it
would be such a headache figuring out fluorescence staining on sectioned
tissues as opposed to cells. Also, do you need to get all the reagent
separate, or can some of the detections kits be used for fluorescence?

Thanks.

 

Renee' Till, HT

 

Arkansas Childrens Nutrition Center

1120 Marshall

Little Rock, AR 

72202

 

(501) 364-2774

 


Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.