[Histonet] flourescent

[Satoshi Akima]

Hi Renee,

I do confocal microscopy rather than on paraffin sections but I can 
tell you this: Try to get a primary conjugated antibody where possible. 
The more steps you have, the more steps that can go wrong. It's also 
less expensive to get a primary conjugated antibody. Many antibodies 
that are made for FACS can also be used for immunofluorescent or 
immunohistochemistry.

Toshi Akima
PhD Student
Centre for Transplantation and Renal Research
Westmead Millenium Institute
Sydney, Australia

On 09/04/2005, at 3:41 AM, Till, Renee wrote:

> Is there a basic protocol for doing fluorescent IHC on frozen or
> paraffin sections? Is it better to use a primary antibody conjugated
> with FITC or whatever, instead of with the secondary? I never thought 
> it
> would be such a headache figuring out fluorescence staining on 
> sectioned
> tissues as opposed to cells. Also, do you need to get all the reagent
> separate, or can some of the detections kits be used for fluorescence?
>
> Thanks.
>
>
>
> Renee' Till, HT
>
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