[Histonet] Universal (protein) block for IHC

Richard Cartun Rcartun <@t> harthosp.org
Thu Apr 7 13:07:47 CDT 2005


I find it interesting that some labs still use a protein or normal sera
block.  We stopped doing this years ago (for paraffin sections) and we
never see any nonspecific staining.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Johnson, Teri" <TJJ <@t> Stowers-Institute.org> 04/06/05 05:34PM >>>
Has anybody converted to using a universal protein block (casein) for
their immunohistochemistry protocols rather than using species
specific
normal sera?  For those who have made the conversion, did you have to
make any changes to your methodology or did you just plug it in to
your
protocol and run?  Did you notice any difference in the staining
intensity for known antibodies (stronger, weaker, no change)?  Are you
also using it as a secondary antibody diluent or for washes?  Finally,
are you using commercially available reagent or are you making your
own?
 
Thanks so much!
 

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org 


 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-----------------------------------------------
Confidentiality Notice

This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged.  Any unauthorized review, use, disclosure, or distribution is prohibited.  If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message.




More information about the Histonet mailing list