[Histonet] Laminin antibody
juan.gutierrez <@t> christushealth.org
Thu Apr 7 08:09:27 CDT 2005
We use the same antibody at 1:20 with proteinase k from DAKO for 10min at RT.
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
My opinions are my own and do not reflect those of my employer. Long live free speech!
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Coolen, Neeltje
Sent: Thursday, April 07, 2005 3:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Laminin antibody
Are there any people who have experience with the following anti-laminin
antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)?
We tried to use this antibody on paraffin-embedded human skin tissue
(formalin-fixed), but it did not work. We tried several antigen
retrieval procedures, like pepsin, proteinase K and trypsin digestion,
but all of these fail.
Does anyone know an appropriate antigen retrieval or should I try
Thank you for your help.
Neeltje Coolen, PhD student
1941 AJ Beverwijk
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet