[Histonet] Laminin antibody

[Dawson, Glen]

I use this antibody @ a 1:25 dilution.  Dako LSAB2 detection.  Dako
Proteinase K enzyme retrieval for 5 minutes & it works very well.

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: Coolen, Neeltje [mailto:ncoolen <@t> burns.nl]
Sent: Thursday, April 07, 2005 2:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Laminin antibody


Dear Histonetters,
 
Are there any people who have experience with the following anti-laminin
antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)?
We tried to use this antibody on paraffin-embedded human skin tissue
(formalin-fixed), but it did not work. We tried several antigen
retrieval procedures, like pepsin, proteinase K and trypsin digestion,
but all of these fail.
Does anyone know an appropriate antigen retrieval or should I try
another antibody? 
 
Thank you for your help.
 
Neeltje Coolen, PhD student
VSBN
Zeestraat 26
1941 AJ Beverwijk
The Netherlands
 
 
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