[Histonet] Laminin antibody

[Sebree Linda A.]

We're using a polyclonal laminin at 1:50 from Neomarkers with pretty
good luck.  Pretreatment with protease.  The stain can be pretty dirty
but we can't back off on the dilution or incubation time or we lose some
of the specific staining.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Coolen,
Neeltje
Sent: Thursday, April 07, 2005 3:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Laminin antibody


Dear Histonetters,
 
Are there any people who have experience with the following anti-laminin
antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We
tried to use this antibody on paraffin-embedded human skin tissue
(formalin-fixed), but it did not work. We tried several antigen
retrieval procedures, like pepsin, proteinase K and trypsin digestion,
but all of these fail. Does anyone know an appropriate antigen retrieval
or should I try another antibody? 
 
Thank you for your help.
 
Neeltje Coolen, PhD student
VSBN
Zeestraat 26
1941 AJ Beverwijk
The Netherlands
 
 
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