[Histonet] Laminin antibody
Edmondson David (RBV) NHS Christie Tr
David.Edmondson <@t> christie-tr.nwest.nhs.uk
Thu Apr 7 06:06:15 CDT 2005
We use have used Protease digestion with success, though we do not use the Antibody much. Protease is Sigma P-8038, Type XXIV. 10mg in 10ml of PBS 20 mins
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Coolen,
Sent: 07 April 2005 09:37
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Laminin antibody
Are there any people who have experience with the following anti-laminin
antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)?
We tried to use this antibody on paraffin-embedded human skin tissue
(formalin-fixed), but it did not work. We tried several antigen
retrieval procedures, like pepsin, proteinase K and trypsin digestion,
but all of these fail.
Does anyone know an appropriate antigen retrieval or should I try
Thank you for your help.
Neeltje Coolen, PhD student
1941 AJ Beverwijk
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