[Histonet] How to eliminate AFB contamination on slides?
llewllew <@t> shaw.ca
Wed Apr 6 14:25:30 CDT 2005
If your method has sections in a coplin jar of carbol fuchsin in an oven at
60ºC for an hour or so, then be aware that this technique has been shown to
be a source of cross contamination. It's unfortunate since it produces good
Another source of contamination is the taps used for washing sections. They
can have a scale built up inside the vacuum trap. The scale may be acid
fast organisms. Eliminate by changing the vacuum trap and regularly
cleaning the insides of the tap with a test tube brush.
----- Original Message -----
From: <Heather.A.Harper <@t> pcola.med.navy.mil>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 06, 2005 10:34 AM
Subject: [Histonet] How to eliminate AFB contamination on slides?
> We are having problems with the AFB stain. Almost everything we do AFB
> stains on has extracellular "AFB positive organisms" without an associated
> tissue response. Do we need to change our stain or use better grade water?
> Anybody who has any ideas or suggestions or has had this problem in the
> past, let me know what you did to correct the problem. Thank you to all in
> Heather A. Harper
> Supervisor of Histology/Morgue
> Naval Hospital of Pensacola, FL
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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