[Histonet] How to eliminate AFB contamination on slides?

Bryan Llewellyn llewllew <@t> shaw.ca
Wed Apr 6 14:25:30 CDT 2005

If your method has sections in a coplin jar of carbol fuchsin in an oven at 
60ºC for an hour or so, then be aware that this technique has been shown to 
be a source of cross contamination.  It's unfortunate since it produces good 

Another source of contamination is the taps used for washing sections.  They 
can have a scale built up inside the vacuum trap.  The scale may be acid 
fast organisms.  Eliminate by changing the vacuum trap and regularly 
cleaning the insides of the tap with a test tube brush.

Bryan Llewellyn

----- Original Message ----- 
From: <Heather.A.Harper <@t> pcola.med.navy.mil>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 06, 2005 10:34 AM
Subject: [Histonet] How to eliminate AFB contamination on slides?

> We are having problems with the AFB stain. Almost everything we do AFB
> stains on has extracellular "AFB positive organisms" without an associated
> tissue response. Do we need to change our stain or use better grade water?
> Anybody who has any ideas or suggestions or has had this problem in the
> past, let me know what you did to correct the problem. Thank you to all in
> advance.
> Heather A. Harper
> Supervisor of Histology/Morgue
> Naval Hospital of Pensacola, FL
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> Histonet <@t> lists.utsouthwestern.edu
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