[Histonet] How to eliminate AFB contamination on slides?

[Heather.A.Harper <@t> pcola.med.navy.mil]

We are having problems with the AFB stain. Almost everything we do AFB
stains on has extracellular "AFB positive organisms" without an associated
tissue response. Do we need to change our stain or use better grade water?
Anybody who has any ideas or suggestions or has had this problem in the
past, let me know what you did to correct the problem. Thank you to all in
advance.

 

Heather A. Harper

Supervisor of Histology/Morgue

Naval Hospital of Pensacola, FL