[Histonet] Help me get rid of autofluorescence

Justin Julander jgjulander <@t> cc.usu.edu
Wed Sep 29 16:06:08 CDT 2004

I have a virus that expresses GFP during replication.  I have fixed tissue 
from animals 
infected with the virus in 4% paraformaldehyde (Paraformaldehyde from Ted 
Pella as a 
16% solution) in water.  I fix tissues for 4 hours, soak 1-2 days in 30% 
sucrose, freeze in 
OCT, and then section on the cryostat.  My control uninfected tissue samples 
have high 
fluorescence.  Is there a way to avoid this autofluorescence?  Keep in mind 
that I have to 
fix the samples to kill the virus (dangerous to man).
Thanks in advance,

Institute for Antiviral Research
Biotechnology Center Rm. 201
4700 Old Main Hill
Utah State University
Logan, UT 84322-4700
Fax (435)797-3959
Ph. (435)797-3643
jgjulander <@t> cc.usu.edu

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