[Histonet] Bouins substitute in Trichrome staining

[hymclab]

PLEASE POST

-----Original Message-----
From: Andrea Grantham [mailto:algranth <@t> u.arizona.edu] 
Sent: Wednesday, September 29, 2004 10:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bouins substitute in Trichrome staining


I attended a workshop at NSH last week on connective tissue staining and 
one of the attendees said they were using citrate buffer instead of bouins 
as a mordant. I couldn't catch up with her after the workshop to ask about 
the protocol. Is anybody out in histoland doing this? Would you mind 
sharing your protocol if you are?
Thanks!!
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html


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