[Histonet] Cresyl violet vs. cresyl violet acetate

Bonner, Janet Janet.Bonner <@t> FLHOSP.ORG
Mon Sep 27 15:24:53 CDT 2004

I find the most important skill is knowing your resources and where to look
for the answer.  

-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu]
Sent: Monday, September 27, 2004 2:36 PM
Cc: histonet <@t> lists.utsouthwestern.edu; lpwenk <@t> sbcglobal.net
Subject: Re: [Histonet] Cresyl violet vs. cresyl violet acetate

Weighing in on this topic .......


>Isn't looking in the histonet almost the same as if looking it up in a
If she knew which book to use and where to find it she would have the 
information by now.

> At least an effort is being made to look in the right place. 
>What do you all think?
She need to be able to use the library, after all she is a graduate 

>I think the fact that Jennifer was able to find us means that she put some
effort into it. 
I don't know how much effort she put into finding us, maybe she just 
used google?

> Shouldn't we reward her?
The knowledge and knowing where to look for it should be the reward. 
There are a lot of resources that, due to their age, will probably never 
be digitalized and available on the internet. Students need to be able 
to use the library. Histonet does not work too well on the weekends or 
when the server is down.
My $0.02 worth.


>I'll keep my answer on hold until we can find a consensus.  Good luck
>Juan C. Gutierrez, HT(ASCP)
>Histology Laboratory Supervisor
>-----Original Message-----
>From: lpwenk <@t> sbcglobal.net [mailto:lpwenk <@t> sbcglobal.net] 
>Sent: Saturday, September 25, 2004 1:41 PM
>To: Jennifer Brielmaier; histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Cresyl violet vs. cresyl violet acetate
>I have a question, concerning your "mystery" bottle -
>Do you mean that the teacher has no idea what it is (other than it might be
>a cresyl-family dye), and would like some idea/help on how to figure out
>what it is?
>Or, is this an assignment, where each student is supposed to find out the
>chemical nature of each of the dyes, and thus be able to chemically prove
>which dye it is. Where you are getting a grade for this.
>Be honest with us.
>If the teacher actually doesn't know, I think the Histonet community would
>be willing to help.
>If this is your homework assignment, I think that the Histonet community
>would be willing to refer you to a text book where you could look up the
>answers yourself, but that we would not be willing to do your homework for
>Sorry if I'm sounding a little edgy. I'm an instructor in histotechnology.
>All of my assignments are designed to teach students about histotechnology.
>But there is more to our field than just science. Some of my assignments
>"team" assignments, where my students can pull together their knowledge and
>abilities to work on the assignment together. Part of what I'm assess is
>ability of each student to contribute to a team. Some of my other
>assignments are "solo", where the person can use book, journals, internet,
>etc., but they cannot get the answers from their classmates or other techs.
>Part of what I'm assessing is the person's ability to find the answer on
>their own, not be told it. I need to know whether a person can
>and troubleshoot on their own.
>Either way, there are text books and web pages out there with the
>information you need about your dyes. I'm sitting here with one of the
>in my lab right now, which has the information.
>So - if your teacher needs the help, I am willing to help and quote from
>If you are supposed to find the answer, I'll let you know the name of the
>book. And you can look it up for yourself (and hopefully learn about this
>book, and learn about other dyes while leafing through this book).
>So, again, be honest, and let me know.
>Peggy A. Wenk, BA, BS, HTL(ASCP)SLS
>Program Director
>School of Histologic Technicians
>School of Histotechnologist
>William Beaumont Hospital
>Royal Oak, MI 48073
>----- Original Message -----
>From: "Jennifer Brielmaier" <jbrielma <@t> yahoo.com>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Sent: Friday, September 24, 2004 11:43 AM
>Subject: [Histonet] Cresyl violet vs. cresyl violet acetate
>>Hello everyone,
>>I am a first-year graduate student in a biopsychology program and am
>enrolled in a basic histology course this semester. Today in class we are
>going to learn how to make cresyl violet stain solutions. Our instructor
>informed us that we have a "mystery" bottle in the lab; it is not known
>whether it is cresyl violet or cresyl violet acetate. Can anyone tell me
>whether there is a simple test that can be performed that will tell us
>solution is in the bottle? Thanks very much.
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu

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