[Histonet] Processing lung tissue after pulmonary function te sts

[Randolph-Habecker, Julie]

Greg,

I agree with the solvent issue. We have already frozen part of the tissue
for frozen sections as well.

Thanks,

Julie

-----Original Message-----
From: Greg Dobbin [mailto:dobbin <@t> upei.ca]
Sent: Monday, September 27, 2004 6:52 AM
To: Randolph-Habecker, Julie
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing lung tissue after pulmonary function
tests


Hi Julie,
It seems to me that a solvent is a solvent is a solvent! The plastic 
beads (polystyrene I suppose?) will dissolve in which ever solvent is 
used to process the tissue. 

Is frozen sectioning out of the question for some reason?
Greg

From:           	"Randolph-Habecker, Julie" <jhabecke <@t> seattlecca.org>
To:             	"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Date sent:      	Mon, 27 Sep 2004 09:11:52 -0700
Subject:        	[Histonet] Processing lung tissue after pulmonary
function tests

> Histonetters,
> 
> I am working with an investigator who is studying lung function after lung
> transplantation. We would like to look at FFPE lung tissue after the
subject
> has had lung function tests which involve fluorescent plastic beads of
> varying size - the largest being 15 microns. I am concerned that the
normal
> processing could melt the beads and cause artifact. Someone in another lab
> suggested that we use Histoclear II rather than Xylene.
> 
> Does anyone have experience in processing tissue like this? Please give me
> some information on how to handle this tissue.
> 
> Thanks!
> 
> Julie
> 
> Julie Randolph-Habecker, Ph.D.
> Experimental Histopathology Shared Resources
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave. N, G1-300
> PO Box 19023
> Seattle, WA 98109-1024
> Tel: (206) 288-1187
> FAX: (206) 288-1345
> jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>
> 
> 
> 
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Happiness is a journey, not a destination.


 This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.