[Histonet] Peptide preparation for antibody validation

[Tan, MinHan]

Good afternoon, fellow Histonetizens:

- I would like to enquire as to the preparation of peptide for the
purposes of antibody validation. 

- We have a rabbit polyclonal antibody against a novel peptide target
made by Invitrogen that has cytoplasmic staining on IHC in formalin
fixed tissue. 

- We do not have any more of that peptide, and we would like to make
some to perform some competitive IHC (pre-incubate peptide + antibody)

- I would like to enquire: what kind of purity of peptide do we need
(70%, 90%, 95%?) and does it need to be amidated?

- If there is anything else I'd need to watch out for, would appreciate
any pointers! :)

Thank you!

Min-Han Tan
Van Andel Institute

 
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