[Histonet] Paraffin embedding

[Pablo Sánchez Quinteiro]

Dear Bonnie,

The tissue is fractured showing numerous cracks or fissures always filled
with paraffin. They are more conspicuous in the periphery but the whole
section is useless.

Thanks for your help.

Pablo


At 01:43 p.m. 21/09/04 -0500, you wrote:
>Please give us details of exactly what is wrong.
>Thanks,
>
>Bonnie Whitaker
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pablo
>Sánchez Quinteiro
>Sent: Tuesday, September 21, 2004 1:20 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Paraffin embedding
>
>Dear listers,
>
>After the summer break I am back to the list with my first lab headache of
>the season.
>
>The first thing I did was replacing all the liquids in the paraffin
>embedder, Hopefully the first embedding (pieces of mouse brain fixed in
>Paraformaldehyde 4%) should be nice, but when cutting the blocks in the
>microtome I have realized that they are dreadfully ruined. I think that the
>fixation is correct as I have processed pieces from the same animal with
>the vibratome and they seem fine.
>
>So probably something is wrong with the embedding. Perhaps with the new
>liquids should I decrease the time of the embedding specially the final
>paraffin steps. Have you got some hint? How long do you suggest? They are
>very small brain pieces from postanatal mice.
>
>Thre paraffin I use is Tissue-Tek III 4502 from SAKURA Have you got some
>input about it?
>
>Thanks in advance
>
>Pablo
>
>
>
>
>
>
>
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