[Histonet] endogenous peroxidase blocking protocol

Ana Florentin anaflorenti <@t> yahoo.com
Thu Sep 16 09:56:28 CDT 2004

Does anybody know a good protocol for blocking the
endogenous peroxidase? 
I am performing immunoperoxidase staining on rat
spleen frozen sections and I have tried to block
endogenous peroxidase with H2O2, 1% and 0.3% in PBS, 
half hour before the first antibody but is seemed to
be a too high concentration, as the tissue was
Thanks for help!

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