[Histonet] endogenous peroxidase blocking protocol

[Ana Florentin]

Hi,
Does anybody know a good protocol for blocking the
endogenous peroxidase? 
I am performing immunoperoxidase staining on rat
spleen frozen sections and I have tried to block
endogenous peroxidase with H2O2, 1% and 0.3% in PBS, 
half hour before the first antibody but is seemed to
be a too high concentration, as the tissue was
destroyed. 
Thanks for help!
anne


	
		
__________________________________
Do you Yahoo!?
New and Improved Yahoo! Mail - 100MB free storage!
http://promotions.yahoo.com/new_mail