[Histonet] endogenous peroxidase blocking protocol

Joe Nocito jnocito <@t> satx.rr.com
Thu Sep 16 21:35:03 CDT 2004

have you tried a 3% in methanol solution? Depending on the number of slides
you have. I used to use 450 mL methanol to 50mLs H2O2 on really bloody
specimens. You might have to perform a time titer like 5 min., 10 min, 15
min.  Good luck

Histology Manager
Pathology Reference Lab
San Antonio, TX

----- Original Message -----
From: "Ana Florentin" <anaflorenti <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 16, 2004 9:56 AM
Subject: [Histonet] endogenous peroxidase blocking protocol

> Hi,
> Does anybody know a good protocol for blocking the
> endogenous peroxidase?
> I am performing immunoperoxidase staining on rat
> spleen frozen sections and I have tried to block
> endogenous peroxidase with H2O2, 1% and 0.3% in PBS,
> half hour before the first antibody but is seemed to
> be a too high concentration, as the tissue was
> destroyed.
> Thanks for help!
> anne
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