[Histonet] rat embryos

[Jeffus, Brandon]

Hello all... im rather new to immunohistochemistry so bear with me.  I'm
attempting to process some rat embryos for staining with a primary Ab
and then a Cy3 labeled secondary for visualization of protein
localization.  I'm wondering if the best processing method would be
frozen sections of the samples or paraffin embedding the samples?  Any
information would be greatly appreciated.  

 

Brandon C Jeffus

Graduate Student