[Histonet] FISH fading

[Bonnie Whitaker]

Hi Histonet!

 

A colleague called to vent some frustrations about FISH today.  This person
is performing FISH, checking the slides, and thinking the results are good.
A few days later when the PI checks the slides, the slides have faded.  Is
there a specific rule of thumb for how much exposure to light (sunlight and
artificial) and how much time can pass without damaging the signal?  Are
anti-fading agents different?  Should he try something other than what the
manufacturer includes in the kit that they are using?

 

Bonnie Whitaker

Lab Manager

Brown & Associates Medical Laboratories

8076 El Rio

Houston, Texas  77054

713-741-6677