[Histonet] embedding of small artery
galinadeyneko <@t> yahoo.com
Mon Sep 13 10:51:14 CDT 2004
Could someone share an experience about embedding of tissue in agarose gel with further embedding in OCT and cryostate sectioning and , maybe, paraffin processing.
( I found very small piece of information about agarose gel in Myneurolab.com).
Our goal is to embed fresh murine common carotid artery and receive ring (circle) of cross sectioned artery on slide. I per fuse intracardial PBS, 10 % formalin, and PBS+OCT in ratio 1:1, after freeze on dry ice harvested artery, but this very small artery still very soft ant thaws momentarily for embedding straight.
Any advices will appreciate.
Novartis, Cardiovascular department
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