[Histonet] IHC Formalin vs frozen

[JOHN PHILLIPS]

Hello fellow "Histotech".

Do you find the time with this workload to do the QC as well?!!!

Your workload shared between two is phenomenal.How many arms have you got? If you have managed to master the application of translocation could you please share this with the rest of us in the real world. 

You must go through tons of anti-perspirant or is this a slight exageration on my part?

Oh and please do not share your work practice secrets with NHS management here in the UK. Super techs like you would put a lot of lab staff out of work.

John.



> ----------
> From: 	JColCLEFA <@t> aol.com[SMTP:JColCLEFA <@t> aol.com]
> Sent: 	11 September 2004 23:37
> To: 	histonet-request <@t> lists.utsouthwestern.edu; histonet <@t> lists.utsouthwestern.edu; georgecole <@t> ev1.net
> Subject: 	[Histonet] IHC Formalin vs frozen
> 
> My lab works like most clinical labs in that all tissue is submitted in 
> fixative for H&E staining first. Once the pathologist sees the H&E sections he or 
> she will determine whether special stains or Immunostains are required. We 
> process about 100,000 surgical cases per year, and I do about 400 Immuno slides a 
> day. It is much easier and more efficient to work with paraffin blocks rather 
> than cut 400 frozen slides each day for our 5 hour TAT requirement.   I've 
> worked with frozen and paraffin processed material extensively, (I also run our 
> muscle biopsy lab) and as a muscle tech I assume you realize how labor 
> intensive it would be to handle such a high volume of varied tissue (bone marrow 
> biopsies, fatty and fibrous breast tissue, cytology specimens etc) in a frozen 
> arena. We also recieve breast tissue and colon tumor tissue from other hospitals 
> and from as far back at 1980 and the standard of care is formalin fixation and 
> paraffin processing. We have done intensive comparison studies (frozen to 
> fixed) and I can assure all my pathologists that   our staining reactions are 
> optimal. In our high volume, low staffing (me and 1 other tech) situation, it's 
> easier to keep as many procedures as similar as possible. Also, lastly, we cut 2 
> micron sections for 40% of our slides and the distortion from freezing+ the 
> thinness of section required eliminate the utility of frozen sectioning. 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn 
gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu
atynt.  Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r
rheolwr systemau wybod drwy ddefnyddio'r manylion isod.  

Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod,
felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain
Cymru.  

Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i 
Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys 
unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau’r Ddeddf 
Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, 
cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru 
ar  www.newalesnhstrust.org.uk


This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed.  If you have received this email in error please notify
the system manager using the details below.  

The contents of this email represent the views of the individual(s)
named above and do not necessarily represent the views of the 
North East Wales NHS Trust.  

Please be aware that, under the terms of the Freedom of Information Act 
2000, the North East Wales NHS Trust may be required to make public the 
content of any emails or correspondence received.  For futher 
information on Freedom of Information, please refer to the North East 
Wales NHS Trust website at www.newalesnhstrust.org.uk

For further assistance, please contact 
system.administrator <@t> new-tr.wales.nhs.uk.