[Histonet] GFP in a fixed brain tissue

Ajai Vyas ajaivyas <@t> gmail.com
Fri Sep 10 20:12:43 CDT 2004


Hi,
   Regards from a new entrants to histonet. I have GFP labelled
parasites sitting in brain of my rats. I need to slice the brain and
locate these parasites. Will GFP still be present if I use fixatives
like formaldehyde or organic solvents like xylene? What fixing
protocols are best for preserving GFP signal?
Thanks in advance,
AJAI




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