[Histonet] IHC on Frozen Sections

[Allen, Rhonda]

Hello,

 

    I am trying to do the T & B cells on frozen section tonsil for my IHC
exam

    due at the end of this month.  I have tried multiple procedures, and
have

    not yet found one that makes me happy, or looks like the one in the
picture

    at the IHC home page.

    If someone could e-mail me their procedures (mostly from cutting to
antibody

    application) it would be greatly appreciated.

    By the way, my cryostat will not cut thinner than 4-6 microns.

    Thanks in advance,

 

 

    Rhonda Allen

    VA Medical Center