[Histonet] IHC on Frozen Sections

Allen, Rhonda Rhonda.Allen2 <@t> med.va.gov
Fri Sep 10 14:30:45 CDT 2004





    I am trying to do the T & B cells on frozen section tonsil for my IHC

    due at the end of this month.  I have tried multiple procedures, and

    not yet found one that makes me happy, or looks like the one in the

    at the IHC home page.

    If someone could e-mail me their procedures (mostly from cutting to

    application) it would be greatly appreciated.

    By the way, my cryostat will not cut thinner than 4-6 microns.

    Thanks in advance,



    Rhonda Allen

    VA Medical Center


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