[Histonet] GFP in a fixed brain tissue

[Gayle Callis]

Maybe, it is probably best to cut frozen sections, fix with 2% 
paraformaldehyde for 1 minute, rinse, and mount with Molecular Probes, 
Probe On antifade mounting media, ready to use.

Go to Clontech website and read Living Colours manual on some of these 
issues.

Their tech services is excellent.  This question has been discussed, even 
recently, on Histonet, you can search the archives at www.histosearch.org

At 07:12 PM 9/10/2004, you wrote:
>Hi,
>    Regards from a new entrants to histonet. I have GFP labelled
>parasites sitting in brain of my rats. I need to slice the brain and
>locate these parasites. Will GFP still be present if I use fixatives
>like formaldehyde or organic solvents like xylene? What fixing
>protocols are best for preserving GFP signal?
>Thanks in advance,
>AJAI
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)