[Histonet] benzyl benzoate clearing agent

[John Chandler]

That speaker might have been Dave Gard from the Biology Department at the
University of Utah.  You can check out his lab at:

http://froglab.biology.utah.edu/next.html-ssi

--John
jchandler <@t> ial-fa.com
970-217-1321

----- Original Message ----- 
From: "Corazon D. Bucana" <bucana <@t> audumla.mdacc.tmc.edu>
To: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, September 10, 2004 8:49 AM
Subject: RE: [Histonet] benzyl benzoate clearing agent


> Thank you for the info.  I heard a talk about confocal microscopy of
> embryos immunolabeled for some protein that I can't remember now but the
> speaker mentioned benzyl benzoate as a clearing agent after the embryos
> were immunolabeled and claims that they are able to create a stack of
> optical sections to a depth of 300 microns .  This is quite a feat because
> most often we only look at 50-100 microns thick sections.  I wrote the
> speaker asking for the procedure but so far I haven't had a response which
> is why I thought somebody at the Histonet will have an idea about this
agent.
> Thanks
> Cora Bucana
>
>
> >Benzyl benzoate (phenyl methyl benzoate)
> >Refractive index 1.5685 to 1.570.
> >Miscible with alcohol, chloroform, ether and xylene.
> >Never heard of it being used for fluorescence microscopy.
> >It has been used as a clearing agent and mountant for thin museum
> >specimens, often with small amount of methyl salicylate added. The
> >problem with benzyl benzoate is that it is intolerant of water so cannot
> >breathe on the specimen while it is being mounted.
> >Hope this helps
> >Barry
> >
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Corazon
> >D. Bucana
> >Sent: Thursday, September 09, 2004 4:15 PM
> >To: Histonet <@t> lists.utsouthwestern.edu
> >Subject: [Histonet] benzyl benzoate clearing agent
> >
> >I would appreciate any information on the use of benzyl alcohol/benzyl
> >benzoate as a clearing agent for tissues.  I understand this could be
> >used
> >in combination with fluorescence staining to image thick sections but I
> >have not seen any reference to it in the literature (Pub Med search).  I
> >
> >hope somebody out there can direct me to a book or journal that mentions
> >
> >this agent.
> >
> >Thanks,
> >
> >Cora Bucana
> >
> >
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>
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