[Histonet] How obtain a Methyl Green counterstain after a Citratepretreatment?

IHC World info <@t> ihcworld.com
Wed Sep 8 19:01:30 CDT 2004

Methyl green is excellent for IHC counterstain when nuclear markers is used.
It will not overstain nucleus. However, some tissues have low affinity to
methyl green. In this case you will get no or weak nuclear staining. Use the
following protocol may help you solve problems if you haven't done so.


Good luck.


-----Original Message-----
From: Rey, Jean-Philippe [mailto:JPR <@t> Stowers-Institute.org]
Sent: Wednesday, September 08, 2004 5:40 PM
To: Histonet request (E-mail)
Subject: [Histonet] How obtain a Methyl Green counterstain after a

Hi everyone,

I try to counterstain some immunostained sections with Methyl green. The one
which was pretreated with Citrate buffer at 121degre Celcius, can't been
stained with Methyl Green.
Does some one have a clue why? And to be able to obtain some Methyl Green
after a slide undergo a Citrate pretreatment?
I will appreciate your help.
Have a nice day

Jean-Philippe Rey
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO  64110
(816) 926-4305

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