[Histonet] How obtain a Methyl Green counterstain after a Citrate pretreatment?

[Rey, Jean-Philippe]

Hi everyone,
 
I try to counterstain some immunostained sections with Methyl green. The one which was pretreated with Citrate buffer at 121degre Celcius, can't been stained with Methyl Green.
Does some one have a clue why? And to be able to obtain some Methyl Green after a slide undergo a Citrate pretreatment?
I will appreciate your help.
Have a nice day

Jean-Philippe Rey 
Research Technician II 
Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, MO  64110 
(816) 926-4305