[Histonet] Pre-cut controls

[Willis, Donna]

We precut our controls and place them in the refrigerator.  We do not dry
them until they are taken out for use.  The only issue we have found is with
Her-2-neu and what we do is only cut enough for 2 weeks use.

Donna Willis
Histology Lab Manager
Harris Methodist Fort Worth, Tx

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fran Lemons
Sent: Friday, September 03, 2004 9:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pre-cut controls


Does anyone know why IHC control slides cannot be cut ahead of time?  I've
done it at other facilities, but I have been told it is not permissible
here, without any explanation.  Any ideas? Also, has anyone ever heard of
refrigerating pre-cut IHC control slides?  One person suggested to me that
they stopped cutting them ahead of time because they were getting
condensation on them & the stains wouldn't work.  Then she informed me that
they were stored in the fridge.  Seems to me the condensation would have
formed upon removing the slides from a cold environment to a room temp. Your
thoughts/theories are appreciated. Fran Walker Histology Technical
Specialist ETBH Knoxville


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