[Histonet] Pre-cut controls

pruegg <@t> ihctech.net pruegg <@t> ihctech.net
Fri Sep 3 17:06:45 CDT 2004

I store all my precious blocks for IHC controls at 4dc and any pre-cut slides are also stored dessicated at 4dc.  There are some antibodies where it doesn't matter and some that are compromised if pre-cut and left at rt for long periods of time so I don't take a chance, but I am in research and have the space to store my tissues at 4dc.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Fran
Sent: Friday, September 03, 2004 7:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pre-cut controls

Does anyone know why IHC control slides cannot be cut ahead of time?  I've done it at other facilities, but I have been told it is not permissible here, without any explanation.  Any ideas?
Also, has anyone ever heard of refrigerating pre-cut IHC control slides?  One person suggested to me that they stopped cutting them ahead of time because they were getting condensation on them & the stains wouldn't work.  Then she informed me that they were stored in the fridge.  Seems to me the condensation would have formed upon removing the slides from a cold environment to a room temp.
Your thoughts/theories are appreciated.
Fran Walker
Histology Technical Specialist
ETBH Knoxville

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