[Histonet] Pre-cut controls
MAUGER <@t> email.chop.edu
Fri Sep 3 09:40:29 CDT 2004
Some antigenicity can be lost when cut slides are stored for a long time. Time varies for different antigens, and some are not effected at all. Some people think refrigeration, or even freezing controls slides lengthens storage time. I personally think that it's best to cut what you will use in a few months time. Trial and error.
>>> "Fran Lemons" <flemons <@t> bhset.org> 09/03/04 10:15AM >>>
Does anyone know why IHC control slides cannot be cut ahead of time? I've done it at other facilities, but I have been told it is not permissible here, without any explanation. Any ideas?
Also, has anyone ever heard of refrigerating pre-cut IHC control slides? One person suggested to me that they stopped cutting them ahead of time because they were getting condensation on them & the stains wouldn't work. Then she informed me that they were stored in the fridge. Seems to me the condensation would have formed upon removing the slides from a cold environment to a room temp.
Your thoughts/theories are appreciated.
Histology Technical Specialist
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