[Histonet] Eosin, phloxine and OG?
bills <@t> icpmr.wsahs.nsw.gov.au
Wed Oct 20 00:12:00 CDT 2004
I haven't heard of Orange G being used before.
But we use an aqueous solution of 1% eosin yellowish and 1% erythrosin B in
0.25% Sodium Hydrogen Carbonate and 2% Magnesium sulphate. We have used
this for more than 40 years with great results all the time. Depending on
the pH of your local water it may require a wash for anywhere between 10
secs to 1 min.
Tissue Pathology, ICPMR
Westmead NSW 2145
Ph 02 9845 7774
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
WWmn916 <@t> aol.com
Sent: Wednesday, 20 October 2004 2:56 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Eosin, phloxine and OG?
Say, is anyone familiar with an eosin/phloxine that mixes into it some OG
(for H+E staining)?
The conversation came up recently to "amp" the three shades of red in H+E
staining. It was the first time I'd ever heard of adding Orange G into
eosin/phloxing solution. Are there any labs doing this?
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