[Histonet] Eosin, phloxine and OG?

[Bill Sinai]

I haven't heard of Orange G being used before.

But we use an aqueous solution of 1% eosin yellowish and 1% erythrosin B in
0.25% Sodium Hydrogen Carbonate and 2% Magnesium sulphate.  We have used
this for more than 40 years with great results all the time.  Depending on
the pH of your local water it may require a wash for anywhere between 10
secs to 1 min.

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
WWmn916 <@t> aol.com
Sent: Wednesday, 20 October 2004 2:56 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Eosin, phloxine and OG?


Hello everyone,
Say, is anyone familiar with an eosin/phloxine that mixes into it some OG
(for H+E staining)?
The conversation came up recently to "amp" the three shades of red in H+E
staining.  It was the first time I'd ever heard of adding Orange G into
eosin/phloxing solution. Are there any labs doing this?

Thanks:-)
Deb
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