[Histonet] Dewaxing in IHC

[Gayle Callis]

We have used both Clearite 3 (xylene substitute) from Richard Allen, and 
xylene.  A key thing is to do enough changes and longer times in these 
solvents to insure good paraffin removal, plus we keep them relatively 
fresh (rotation is done more frequently to insure first solvent does not 
contain lots of paraffin aka contaminated after many sections going through 
prior to IHC slides.

We do 3 change, 5 minutes per change and either solvent for paraffin 
removal has worked well, no complaints from those doing IHC on paraffin 
sections.

For all other staining, we use Clearite 3, 2 changes at 3 minutes each, but 
once again, keep reagents rotated.  Limonene makes too many people sick in 
our lab, not allowed on the premises.


At 08:26 AM 10/2/2004, you wrote:
>Dear histonetters
>I would like to hear, what reagens is the most used in IHC for dewaxing 
>the slides.
>We use xylen. And there is the opinion in our lab, that xylen is superior 
>to other xylen-substitutes. Do you agree?
>For the HE-slides we use limonen, but for any delicate stain the boss 
>demands xylen.
>thanks
>Gudrun
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)