[Histonet] Dewaxing in IHC

[esteban enriquez]

Best thing I've heard used for dewaxing on certain
systems - which can also pull double duty as a cleaner
in tissue processors - is Isopar-L from Exxon-Mobil.
Dirt cheap by the 5 gallon tin or 55 gallon drum. This
is what Vision Biosystem use for dewax on their
Bondmax and as a cleaner on their Peloris.

Esteban


--- Phillip Huff <histophilhuff <@t> yahoo.com> wrote:

> We are currently using EZ-dewax from bioGenex. It
> works great, is re-usable until the solution is
> saturated with wax and is very good for the tissues.
>  
> http://www.biogenex.net/profile.php?pagename=autant1
>  
> Phil
> 
> Gudrun Lang <gu.lang <@t> gmx.at> wrote:
> Dear histonetters
> I would like to hear, what reagens is the most used
> in IHC for dewaxing the slides.
> We use xylen. And there is the opinion in our lab,
> that xylen is superior to other xylen-substitutes.
> Do you agree?
> For the HE-slides we use limonen, but for any
> delicate stain the boss demands xylen.
> thanks
> Gudrun
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