[Histonet] HELP!!! - Autofluorescence in the mouse brain - HELP!!!!

[Pawel,Pasierbek]

Dear All,

 

I need you help. One of the users of our microscopy facility is doing
immunoassaying on mouse brain slices. After PFA fixation he sees
autofluorescence from certain regions of the brain. It looks like rather
big grains in the cytoplasm. We see it in the FITC and Cy3 channel!
Unfortunately it is also visible in the unfixed preps.

 

I would be very grateful for any hint how to get rid of it. It is
important that he still is able to do immunostainigs on the process
samples.

 

Thank you very much and have a nice day. 

 

Best regards from Vienna,

Pawel

 

---------------------------------------------------------------

Pawel PASIERBEK, PhD

Institute of Molecular Pathology (IMP)

Biooptics Department

Dr. Bohr-Gasse 7

A-1030 Vienna

AUSTRIA

 

Tel. +43 1 797 30 591

Fax +43 1 798 71 53

e-mail: pawel.pasierbek <@t> imp.univie.ac.at
<mailto:pawel.pasierbek <@t> imp.univie.ac.at>