[Histonet] RE: ki67

[C.M. van der Loos]

Dear Veronique,
We got very good results using the new rabbit monoclonal Ki67, SP6 from Labvision/Neomarkers. Fix your cryostat sections with buffered formalin for 5 min at room temperature and wash with TBS or PBS. Block endogenous peroxidase and start staining as usual. A two step EnVision or PowerVision detection will do. 

Acetone-fixation will cause an ugly diffuse staining of the proliferating nuclei. ]Good luck with staining!

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands


----- Original Message ----- 
>From  "Veronique Andriessen" <vandries <@t> vub.ac.be> 
Date  Wed, 26 May 2004 14:03:01 +0200 
To  "Histonet" <histonet <@t> lists.utsouthwestern.edu> 
Subject  [Histonet] ki67 
Hello histonetters,

I'm searching for a Ki67 antibody that will work on frozen sections of rat
pancreas. Can you give me some advice?

Best regards,

Veronique Andriessen BAS
Lab. Molecular Liver Cell Biology,
Free University Brussels (VUB)
Belgium