[Histonet] RE: ki67
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Wed May 26 13:43:38 CDT 2004
We got very good results using the new rabbit monoclonal Ki67, SP6 from Labvision/Neomarkers. Fix your cryostat sections with buffered formalin for 5 min at room temperature and wash with TBS or PBS. Block endogenous peroxidase and start staining as usual. A two step EnVision or PowerVision detection will do.
Acetone-fixation will cause an ugly diffuse staining of the proliferating nuclei. ]Good luck with staining!
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands
----- Original Message -----
>From "Veronique Andriessen" <vandries <@t> vub.ac.be>
Date Wed, 26 May 2004 14:03:01 +0200
To "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Subject [Histonet] ki67
I'm searching for a Ki67 antibody that will work on frozen sections of rat
pancreas. Can you give me some advice?
Veronique Andriessen BAS
Lab. Molecular Liver Cell Biology,
Free University Brussels (VUB)
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