[Histonet] Immuno slide problem
anissachoi <@t> hotmail.com
Wed May 26 14:04:19 CDT 2004
Thanks to all who replied to my question. Please see if the following will
help to gain new insight
into my problem.
1. The red box is on the bottom of our slides. I do not think it would
interfere with the vortex
action of the stainer. If the vortex mixing is not working or weak, I
would expect the section
on the bottom end of the slide not stained as reagent is dispensed onto
the red box area on
Ventana stainer. Unlike the Ventana Benchmark with slides lying directly
flat on the tray. our
slides are clipped on both ends with spaces between slides and the tray.
2.The problem is not repeating. We recut using slides from the same batch
and everthing was fine.
The other day, out of a run of 20 slides, 2 slides did not work( both
control and test did not
work).We checked dispenser, AEC kit etc and could not find anything
wrong. We repeated
without altering anything, same control, same AEC kit, same protease ,
same antibody dispenser
and slides from same batch. The repeated run was fine.
3. Ventana suggested the red paint may get onto some slides and causes
patchy and uneven
staining. I was told that the particles from the red stuff affect the
staining reaction on the
4. As I have mentioned in my last e-mail, I put the origional problem
slides( the skin section in the
middle did not get stained while the control and the other section
worked just fine) back on
the stainer and ran it again after the stainer was thoroughtly checked
and serviced by Ventana.
I still could not get the section in the middle stained.
5. Is it reasonable to conclude that we have a slide problem ( occasional
defective slides in the
batch), rather than a machine problem ? Would you switch to other type
of charged slides?
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